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MLH1 Polyclonal antibody

MLH1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 11697-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

MutL protein homolog 1, HNPCC2, HNPCC, hMLH1, FCC2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, human testis tissue, HEK-293 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11697-1-AP targets MLH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLH1 fusion protein Ag2319 相同性解析による交差性が予測される生物種
Full Name mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Calculated molecular weight 756 aa, 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC006850
Gene Symbol MLH1
Gene ID (NCBI) 4292
RRIDAB_2145604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40692
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.

Protocols

Product Specific Protocols
WB protocol for MLH1 antibody 11697-1-APDownload protocol
IF protocol for MLH1 antibody 11697-1-APDownload protocol
IP protocol for MLH1 antibody 11697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Metab

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer

Authors - Yu Jiang
mouseIF

Nucleic Acids Res

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Authors - Lu Chen
zebrafishWB

Nucleic Acids Res

Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.

Authors - Jingzhen Li
humanIHC

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
humanWB

Nucleic Acids Res

Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.

Authors - Yi Yuan
humanIHC

Cancer Lett

Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination

Authors - Shujia Chen
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