Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
25298-1-AP targets MLH3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MLH3 fusion protein Ag18201 相同性解析による交差性が予測される生物種 |
| Full Name | mutL homolog 3 (E. coli) |
| Calculated molecular weight | 1453 aa, 164 kDa |
| Observed molecular weight | 164 kDa |
| GenBank accession number | BC112167 |
| Gene Symbol | MLH3 |
| Gene ID (NCBI) | 27030 |
| RRID | AB_2880015 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHC1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MLH3 antibody 25298-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.
| ||
Prog Neurobiol Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice |