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  • Featured Product
  • KD/KO Validated

MLK3 Polyclonal antibody, PBS Only

MLK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11996-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLK3 Polyclonal antibody (11996-1-AP)

    WB analysis using 11996-1-AP (same clone as 11996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11996-1-AP (MLK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MLK3 Polyclonal antibody (11996-1-AP)

    WB analysis of HEK-293 using 11996-1-AP (same clone as 11996-1-PBS)

    WB result of MLK3 antibody (11996-1-AP, 1:500) with si-Control and si-MLK3 transfected HEK293 cells. This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLK3 Polyclonal antibody (11996-1-AP)

    IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLK3 Polyclonal antibody (11996-1-AP)

    IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLK3 Polyclonal antibody (11996-1-AP)

    IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLK3 Polyclonal antibody (11996-1-AP)

    IHC staining of human colon cancer using 11996-1-AP (same clone as 11996-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11996-1-AP (MLK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using MLK3 Polyclonal antibody (11996-1-AP)

    IF Staining of NIH/3T3 using 11996-1-AP (same clone as 11996-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLK3 antibody (11996-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLK3 Polyclonal antibody (11996-1-AP)

    IF Staining of HeLa using 11996-1-AP (same clone as 11996-1-PBS)

    Immunofluorescent analysis of Hela cells, using MAP3K11 antibody 11996-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11996-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11996-1-PBS targets MLK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLK3 fusion protein Ag2636 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase kinase 11
Calculated molecular weight 847 aa, 93 kDa
Observed molecular weight93 and 65 kDa
GenBank accession numberBC011263
Gene symbol MLK3
Gene ID (NCBI) 4296
RRIDAB_10694137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MLK3 (Mixed lineage kinase 3) is also named as MAP3K11 and activates the JUN N-terminal pathway. It influences microtubule organization during the cell cycle. Mouse epididymal sperm contained an 60 kDa variant of MLK3, which may have been a result of proteolytic cleavage of the longer 93 kDa form seen in many tissues and the 50 kDa band is not known and not infrequently observed(PMID:17377140). Exposure of cells to LPS resulted in an increase in MLK3 dimers(of approximately 220 kDa), whereas inactivation of GSK-3b by TWS119 blocked MLK3 dimerization(PMID:21194439).