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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLL4 Polyclonal antibody (31396-1-AP)

    WB analysis using 31396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31396-1-AP (MLL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MLL4 Polyclonal antibody (31396-1-AP)

    IF Staining of U2OS using 31396-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MLL4 antibody (31396-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MLL4 Polyclonal antibody

MLL4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31396-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

MLL1B, KMT2B, KIAA0304, HRX2, DYT28

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31396-1-AP targets MLL4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLL4 fusion protein Ag35385 相同性解析による交差性が予測される生物種
Full Name myeloid/lymphoid or mixed-lineage leukemia 4
Observed molecular weight180kDa;320kDa
GenBank accession numberBC009337
Gene Symbol MLL4
Gene ID (NCBI) 9757
RRIDAB_3669963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UMN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MLL4, also known as KMT2B, which is an essential subunit of the H3K4 methylation complexes, regulates cell type-specific transcriptional programs through enhancer activation. Taspase 1 mediates cleavage of the MLL protein, generating 320 kDa N-terminal (MLL-N) and 180 kDa C-terminal (MLL-C) fragments (PMID: 24213472).

Protocols

Product Specific Protocols
WB protocol for MLL4 antibody 31396-1-APDownload protocol
IF protocol for MLL4 antibody 31396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols