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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using MLPH Polyclonal antibody (10338-1-AP)

    WB analysis of A375 using 10338-1-AP (same clone as 10338-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 10338-1-AP (Melanophilin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A375 cells using MLPH Polyclonal antibody (10338-1-AP)

    IP experiment of A375 using 10338-1-AP (same clone as 10338-1-PBS)

    IP result of anti-Melanophilin (IP:10338-1-AP, 4ug; Detection:10338-1-AP 1:600) with A375 cells lysate 3600ug. This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Polyclonal antibody (10338-1-AP)

    IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MLPH Polyclonal antibody (10338-1-AP)

    IHC staining of human skin cancer using 10338-1-AP (same clone as 10338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10338-1-AP (Melanophilin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLPH Polyclonal antibody (10338-1-AP)

    IF Staining of HeLa using 10338-1-AP (same clone as 10338-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10338-1-AP (Melanophilin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MLPH Polyclonal antibody (10338-1-AP)

    IF Staining of MCF-7 using 10338-1-AP (same clone as 10338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Melanophilin antibody (10338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10338-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MLPH Polyclonal antibody, PBS Only

MLPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10338-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10338-1-PBS targets MLPH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLPH fusion protein Ag0316 相同性解析による交差性が予測される生物種
Full Name melanophilin
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa, 80 kDa
GenBank accession numberBC001653
Gene Symbol MLPH
Gene ID (NCBI) 79083
RRIDAB_2146104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV36
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.