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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MLX Monoclonal antibody

MLX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A8D2

Cat no : 67042-1-Ig

Synonyms

bHLHd13, MAD7, Max like bHLHZip protein, MAX like protein X, MLX, MXD7, Protein BigMax, TCFL4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLX Monoclonal antibody (67042-1-Ig)

    WB analysis using 67042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67042-1-Ig (MLX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human breast cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human liver cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human liver cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MLX Monoclonal antibody (67042-1-Ig)

    IHC staining of human breast cancer using 67042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67042-1-Ig (MLX antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLX Monoclonal antibody (67042-1-Ig)

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MLX Monoclonal antibody (67042-1-Ig)

    IF Staining of HepG2 using 67042-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MLX antibody (67042-1-Ig, Clone: 2A8D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67042-1-Ig targets MLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MLX fusion protein Ag28729 相同性解析による交差性が予測される生物種
Full Name MAX-like protein X
Calculated molecular weight 298 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010689
Gene symbol MLX
Gene ID (NCBI) 6945
RRIDAB_2882356
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MLX antibody 67042-1-IgDownload protocol
IHC protocol for MLX antibody 67042-1-IgDownload protocol
IF protocol for MLX antibody 67042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols