• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MLXIPL Polyclonal antibody, PBS Only

MLXIPL Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13256-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using MLXIPL Polyclonal antibody (13256-1-AP)

    WB analysis of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MLXIPL Polyclonal antibody (13256-1-AP)

    WB analysis using 13256-1-AP (same clone as 13256-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MLXIPL Polyclonal antibody (13256-1-AP)

    WB analysis of HeLa using 13256-1-AP (same clone as 13256-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using MLXIPL Polyclonal antibody (13256-1-AP)

    WB analysis of human liver using 13256-1-AP (same clone as 13256-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MLXIPL Polyclonal antibody (13256-1-AP)

    WB analysis of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13256-1-AP (MLXIPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MLXIPL Polyclonal antibody (13256-1-AP)

    IP experiment of HepG2 using 13256-1-AP (same clone as 13256-1-PBS)

    IP result of anti-MLXIPL (IP:13256-1-AP, 4ug; Detection:13256-1-AP 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 13256-1-PBS in a different storage buffer formulation.

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13256-1-PBS targets MLXIPL in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLXIPL fusion protein Ag3481 相同性解析による交差性が予測される生物種
Full Name MLX interacting protein-like
Calculated molecular weight 852 aa, 93 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC012925
Gene symbol MLXIPL
Gene ID (NCBI) 51085
RRIDAB_2266665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Carbohydrate response element-binding protein (ChREBP), also known as MLXIPL, is a glucose-responsive transcription factor that has a fundamental role in the glucose-mediated induction of genes involved in hepatic glycolysis and lipogenesis. Circulating blood glucose levels affect ChREBP activity in hepatocytes largely by post-translational mechanisms that include phosphorylation-dependent subcellular localization [PMID:21665952]. It enhances expression of the liver pyruvate kinase (LPK) gene and all lipogenic enzyme genes resulting in the conversion of excess dietary carbohydrate into triglycerides that can be stored as fat [PMID:15118080]. Molecular Weight of ChREBP splice variants: 62/78/91/93 kDa.