Neprilysin/CD10 Polyclonal antibody
Neprilysin/CD10 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, HEK-293 cells, rat kidney tissue |
Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue, human liver cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
10302-1-AP targets Neprilysin/CD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, hamster |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Neprilysin/CD10 fusion protein Ag0427 相同性解析による交差性が予測される生物種 |
Full Name | membrane metallo-endopeptidase |
Calculated molecular weight | 750 aa, 86 kDa |
Observed molecular weight | 90-100 kDa |
GenBank accession number | BC101658 |
Gene symbol | CD10 |
Gene ID (NCBI) | 4311 |
RRID | AB_10640893 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).
Protocols
Product Specific Protocols | |
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WB protocol for Neprilysin/CD10 antibody 10302-1-AP | Download protocol |
IHC protocol for Neprilysin/CD10 antibody 10302-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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J Pathol Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion. | ||
Fitoterapia A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Luca (Verified Customer) (09-12-2022) | The antibody unfortunately did not work but most likely the conditions of the WB need optimized.
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FH Luca (Verified Customer) (06-10-2022) | I unfortunately could not detect a band against this protein even when expressed using a plasmid, but I have to better optimize my conditions. I liked having the facts sheet with me as that was helpful, so thank you for including it with the Ab.
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FH Boyan (Verified Customer) (10-27-2021) | This antibody recognised a clean band around 100 kd.
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