Neprilysin/CD10 Polyclonal antibody

Name: Neprilysin/CD10 Polyclonal antibody
Brand: Proteintech
SKU: 18008-1-AP
Rating: 1.00 (1 reviews)

Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 18008-1-AP

Synonyms

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

Validation Data Gallery

Filter:

AllWBIPIHCIF-P

WB analysis of mouse kidney using 18008-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 18008-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 18008-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 18008-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 18008-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 18008-1-AP
IP result of anti-Neprilysin/CD10 (IP:18008-1-AP, 3ug; Detection:18008-1-AP 1:500) with Raji cells lysate 2400ug.
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 18008-1-AP (MME,CD10 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human renal cell carcinoma using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human renal cell carcinoma using 18008-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of human kidney using 18008-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human kidney using 18008-1-AP
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 18008-1-AP (Neprilysin/CD10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 18008-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, human kidney tissue, rat kidney tissue
Positive IP detected in	Raji cells
Positive IHC detected in	human kidney tissue, human renal cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 17 publications below
IHC	See 5 publications below
IF	See 2 publications below

Product Information

18008-1-AP targets Neprilysin/CD10 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Neprilysin/CD10 fusion protein Ag12506 相同性解析による交差性が予測される生物種
Full Name	membrane metallo-endopeptidase
Calculated molecular weight	80aa,9 kDa; 750aa,85 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC106070
Gene symbol	CD10
Gene ID (NCBI)	4311
RRID	AB_2146541
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
WB protocol for Neprilysin/CD10 antibody 18008-1-AP	Download protocol
IHC protocol for Neprilysin/CD10 antibody 18008-1-AP	Download protocol
IF protocol for Neprilysin/CD10 antibody 18008-1-AP	Download protocol
IP protocol for Neprilysin/CD10 antibody 18008-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression Authors - Rui Zhou View Article
human	WB	Brain MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation. Authors - Bing-Lin Zhu View Article
human,mouse	WB	Aging Cell Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease Authors - Guoxin Zhang View Article
human	WB	Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. Authors - Hui Zeng View Article
mouse	WB	Mol Neurobiol Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression. Authors - Yasuteru Inoue View Article
human	IHC	BMC Genomics Genetic control of renal tumorigenesis by the mouse Rtm1 locus. Authors - Jensen José Ricardo JR View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Boyan (Verified Customer) (10-04-2021)

calculated size is 85 kd. This antibody recognized major bands at 60, 30 kd.

Applications: Western Blot

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neprilysin/CD10 Polyclonal antibody

Neprilysin/CD10 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis of mouse kidney using 18008-1-AP

WB analysis using 18008-1-AP

WB analysis of mouse kidney using 18008-1-AP

WB analysis of human kidney using 18008-1-AP

WB analysis of rat kidney using 18008-1-AP

IP experiment of Raji using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human kidney using 18008-1-AP

IHC staining of human renal cell carcinoma using 18008-1-AP

IHC staining of human renal cell carcinoma using 18008-1-AP

IF Staining of human kidney using 18008-1-AP

IF Staining of human kidney using 18008-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Genetic control of renal tumorigenesis by the mouse Rtm1 locus.

Reviews

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