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Validation Data Gallery

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  • Sample test of MP51000-1

    Sample test of MP51000-1

    Cytometric bead array of A549 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. A549 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Sample test of MP51000-1

    Sample test of MP51000-1

    Cytometric bead array of MM231 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. MM231 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Sample test of MP51000-1

    Sample test of MP51000-1

    Cytometric bead array of U2OS cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. U2OS cells were cultured for 2 days in normal culture media or PMA (200nM, 48h) containing media. 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

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MMP-9 Monoclonal antibody, PBS Only (Detector)

MMP-9 Monoclonal Antibody for Indirect ELISA, Sample test
Cat No. 60600-2-PBS
Clone No.1E5G7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, Sample test

Synonyms

MMP9, GELB, EC:3.4.24.35, CLG4B, 82 kDa matrix metalloproteinase-9

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60600-2-PBS targets MMP-9 as part of a matched antibody pair:

MP51000-1: 60600-1-PBS capture and 60600-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Calculated molecular weight 707 aa, 78 kDa
GenBank accession numberBC006093
Gene Symbol MMP-9
Gene ID (NCBI) 4318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14780
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.