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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP11 Polyclonal antibody

MMP11 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30615-1-AP

Synonyms

Matrix metalloproteinase 11, MMP 11, MMP11, SL 3, ST3, STMY3, Stromelysin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP11 Polyclonal antibody (30615-1-AP)

    WB analysis using 30615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30615-1-AP (MMP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP11 Polyclonal antibody (30615-1-AP)

    IF Staining of A549 using 30615-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MMP11 Polyclonal antibody (30615-1-AP)

    IF Staining of MCF-7 using 30615-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MMP11 antibody (30615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, T-47D cells
Positive IF/ICC detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30615-1-AP targets MMP11 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP11 fusion protein Ag31191 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 11 (stromelysin 3)
Calculated molecular weight 55 kDa
Observed molecular weight50-55 kDa
GenBank accession numberNM_005940
Gene symbol MMP11
Gene ID (NCBI) 4320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinase 11 (MMP11), also named stromelysin-3, is a member of the stromelysin subgroup belonging to MMPs superfamily. The role of MMP11 in cancer progression has been shown by several preclinical observations: its expression promotes tumor take in mice, homing of malignant epithelial cells, cancer progression by remodeling extracellular matrix, and antiapoptotic and antinecrotic effect on tumor cells (PMID: 19509157, 26892540). MMP11 has a calculated molecular mass of 55 kDa.

Protocols

Product Specific Protocols
WB protocol for MMP11 antibody 30615-1-APDownload protocol
IF protocol for MMP11 antibody 30615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols