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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP12 Monoclonal antibody (66930-1-Ig)

    WB analysis using 66930-1-Ig (same clone as 66930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66930-1-Ig (MMP12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66930-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using MMP12 Monoclonal antibody (66930-1-Ig)

    WB analysis of A549 using 66930-1-Ig (same clone as 66930-1-PBS)

    WB result of MMP12 antibody (66930-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP12 transfected A549 cells. This data was developed using the same antibody clone with 66930-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MMP12 Monoclonal antibody, PBS Only

MMP12 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66930-1-PBS
Clone No.1C6B5

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66930-1-PBS targets MMP12 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMP12 fusion protein Ag19068 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 12 (macrophage elastase)
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 54 kDa, 45 kDa
GenBank accession numberBC112301
Gene Symbol MMP12
Gene ID (NCBI) 4321
RRIDAB_2882256
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP39900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).