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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP13 Recombinant antibody, PBS Only

MMP13 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240060A1

Cat no : 83188-4-PBS

Synonyms

Matrix metalloproteinase-13, Matrix metalloproteinase 13, EC:3.4.24.-, Collagenase 3, CLG3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP13 Recombinant antibody (83188-4-RR)

    WB analysis using 83188-4-RR (same clone as 83188-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83188-4-RR (MMP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83188-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using MMP13 Recombinant antibody (83188-4-RR)

    FC experiment of MCF-7 using 83188-4-RR (same clone as 83188-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-4-RR, Clone:240060A1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83188-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83188-4-RR

    Affinity and Kinetic Characterization of 83188-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-4-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83188-4-PBS is MMP13 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MMP13 fusion protein Ag34892 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 13
Calculated molecular weight 471 aa, 54 kDa
Observed molecular weight 70 kDa, 54 kDa
GenBank accession numberBC074808
Gene symbol MMP13
Gene ID (NCBI) 4322
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated form (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).