MMP14 / MT1-MMP Polyclonal antibody, PBS Only
MMP14 / MT1-MMP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, Indirect ELISA
Conjugate
Unconjugated
Cat no : 14552-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
14552-1-PBS targets MMP14 / MT1-MMP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MMP14 / MT1-MMP fusion protein Ag5965 相同性解析による交差性が予測される生物種 |
Full Name | matrix metallopeptidase 14 (membrane-inserted) |
Calculated molecular weight | 66 kDa |
Observed molecular weight | 66 kDa, 45-50 kDa |
GenBank accession number | BC064803 |
Gene symbol | MMP14 |
Gene ID (NCBI) | 4323 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).