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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP2 Recombinant antibody

MMP2 Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240131F5

Cat no : 81515-2-RR

Synonyms

72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MONA, TBE 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMP2 Recombinant antibody (81515-2-RR)

    IF Staining of HepG2 using 81515-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP2 antibody (81515-2-RR, Clone: 240131F5 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity experiments of 81515-2-RR

    Affinity experiments of 81515-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81515-2-RR against Human MMP2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 81515-2-RR is MMP2 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MMP2 fusion protein Ag0549 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Calculated molecular weight 72 kDa
Observed molecular weight 62-72 kDa
GenBank accession numberBC002576
Gene symbol MMP2
Gene ID (NCBI) 4313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).

Protocols

Product Specific Protocols
IF protocol for MMP2 antibody 81515-2-RRDownload protocol
FC protocol for MMP2 antibody 81515-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols