Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
55467-1-AP targets MMP20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 20 |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | NM_004771 |
| Gene Symbol | MMP20 |
| Gene ID (NCBI) | 9313 |
| RRID | AB_11182261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60882 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
MMP20 belongs to the peptidase M10A family. It degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). MMP20 may play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). This antibody is specific to MMP20.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP20 antibody 55467-1-AP | Download protocol |
| IHC protocol for MMP20 antibody 55467-1-AP | Download protocol |
| IF protocol for MMP20 antibody 55467-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta. | ||
Front Genet Crucial Roles of microRNA-16-5p and microRNA-27b-3p in Ameloblast Differentiation Through Regulation of Genes Associated With Amelogenesis Imperfecta. | ||
Cell Prolif Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA | ||
Biochim Biophys Acta Mol Basis Dis A novel Cdc42-YAP-fibronectin signaling axis regulates ameloblast differentiation during early enamel formation | ||
J Cell Physiol KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis |