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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMP23B Polyclonal antibody

MMP23B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13020-1-AP

Synonyms

Matrix metalloproteinase 23, Matrix metalloproteinase 22, Matrix metalloproteinase 21, matrix metallopeptidase 23B, Femalysin

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MMP23B Polyclonal antibody (13020-1-AP)

    WB analysis of A549 using 13020-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using MMP23B Polyclonal antibody (13020-1-AP)

    WB analysis of PC-3 using 13020-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MMP23B Polyclonal antibody (13020-1-AP)

    WB analysis of HepG2 using 13020-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MMP23B Polyclonal antibody (13020-1-AP)

    WB analysis of A549 using 13020-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MMP23B Polyclonal antibody (13020-1-AP)

    WB analysis of HepG2 using 13020-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MMP23B Polyclonal antibody (13020-1-AP)

    WB analysis of mouse liver using 13020-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13020-1-AP (MMP23B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MMP23B Polyclonal antibody (13020-1-AP)

    IHC staining of human ovary tumor using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using MMP23B Polyclonal antibody (13020-1-AP)

    IHC staining of human ovary tumor using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MMP23B Polyclonal antibody (13020-1-AP)

    IHC staining of human liver cancer using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MMP23B Polyclonal antibody (13020-1-AP)

    IHC staining of human liver cancer using 13020-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13020-1-AP (MMP23B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MMP23B Polyclonal antibody (13020-1-AP)

    IF Staining of HepG2 using 13020-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP23B antibody (13020-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, mouse liver tissue, PC-3 cells
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13020-1-AP targets MMP23B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP23B fusion protein Ag4113 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 23B
Calculated molecular weight 390 aa, 44 kDa
Observed molecular weight 46-60 kDa
GenBank accession numberBC025719
Gene symbol MMP23B
Gene ID (NCBI) 8510
RRIDAB_2146152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP23B(matrix metallopeptidase 23B) is also named as MMP21, MMP22.It is a regulator in matrix remodeling and may be playing a role in enchondral bone formation and in regulation of cranial suture closure .It has 3 isoforms produced by alternative splicing with the molecular weight of 44KDa,48KDa and 46KDa,respectively.It also can be N-glycosylated.

Protocols

Product Specific Protocols
WB protocol for MMP23B antibody 13020-1-APDownload protocol
IHC protocol for MMP23B antibody 13020-1-APDownload protocol
IF protocol for MMP23B antibody 13020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
View Article