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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP24 Polyclonal antibody (30102-1-AP)

    WB analysis using 30102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30102-1-AP (MMP24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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MMP24 Polyclonal antibody

MMP24 Polyclonal Antibody for WB, ELISA
Cat No. 30102-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

Matrix metalloproteinase 24, MMP 24, MMP24, MMP25, MT MMP 5, MT MMP5, MT5 MMP, MT5MMP, MTMMP5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30102-1-AP targets MMP24 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP24 fusion protein Ag31597 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 24 (membrane-inserted)
Calculated molecular weight73KD
Observed molecular weight55-58 kDa
GenBank accession numberNM_006690
Gene Symbol MMP24
Gene ID (NCBI) 10893
RRIDAB_3086228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5R2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP24 (MT5-MMP) is a membrane type matrix metalloproteinase attached to the cell membrane by a transmembrane domain (PMID: 30654475). MMP24 is mainly expressed in the nervous system, hippocampus, and cerebellum (PMID: 10727639,35177031). MMP24 may negatively regulate the aggressiveness of cancer cells under the stiff ECM environment during tumor progression (PMID: 32093160). MMP24 can be detected as 62-65 kDa and 55-58 kDa (PMID: 16495457,20942921,10622708).

Protocols

Product Specific Protocols
WB protocol for MMP24 antibody 30102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols