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  • Featured Product
  • KD/KO Validated

MMP3 Polyclonal antibody

MMP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17873-1-AP

Synonyms

Stromelysin-1, SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP3 Polyclonal antibody (17873-1-AP)

    WB analysis using 17873-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MMP3 Polyclonal antibody (17873-1-AP)

    WB analysis of HeLa using 17873-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MMP3 Polyclonal antibody (17873-1-AP)

    WB analysis of A549 using 17873-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using MMP3 Polyclonal antibody (17873-1-AP)

    WB analysis of A2780 using 17873-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MMP3 Polyclonal antibody (17873-1-AP)

    WB analysis of HeLa using 17873-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MMP3 Polyclonal antibody (17873-1-AP)

    WB analysis of human skeletal muscle using 17873-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP3 Polyclonal antibody (17873-1-AP)

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP3 Polyclonal antibody (17873-1-AP)

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP3 Polyclonal antibody (17873-1-AP)

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP3 Polyclonal antibody (17873-1-AP)

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP3 Polyclonal antibody (17873-1-AP)

    IHC staining of human stomach cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP3 Polyclonal antibody (17873-1-AP)

    IHC staining of human stomach cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP3 Polyclonal antibody (17873-1-AP)

    IF Staining of A549 using 17873-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (17873-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP3 Polyclonal antibody (17873-1-AP)

    IF Staining of A549 using 17873-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (17873-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Tested Applications

Positive WB detected inA2780 cells, A549 cells, HeLa cells, human skeletal muscle tissue, U-87 MG cells, PC-12 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17873-1-AP targets MMP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP3 fusion protein Ag12359 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074869
Gene symbol MMP3
Gene ID (NCBI) 4314
RRIDAB_2146587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Protocols

Product Specific Protocols
WB protocol for MMP3 antibody 17873-1-APDownload protocol
IHC protocol for MMP3 antibody 17873-1-APDownload protocol
IF protocol for MMP3 antibody 17873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB,IF

Autophagy

Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration

Authors - Di Wang
View Article
humanIHC

Bone Res

FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis.

Authors - Dong Guo
View Article
humanWB

Mater Today Bio

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Authors - Zhanqiu Dai
View Article
ratWB

Phytomedicine

Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells

Authors - Dong Wang
View Article
humanWB

Clin Cancer Res

BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.

Authors - Zebing Liu
View Article
mouse

Gut Microbes

Gut microbiota regulate tumor metastasis via circRNA/miRNA networks.

Authors - Zhuxian Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ana Luísa (Verified Customer) (07-25-2022)

Permeabilization: 0,3% Triton X-100 Blocking 2,5% BSA + 0,1% Triton X-100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Intervertebral disc cells