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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis of HeLa using 66338-1-Ig (same clone as 66338-1-PBS)

    WB result of MMP3 antibody (66338-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP3 transfected HeLa cells. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis of pig heart using 66338-1-Ig (same clone as 66338-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caco-2 cells using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis of Caco-2 using 66338-1-Ig (same clone as 66338-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis of human heart using 66338-1-Ig (same clone as 66338-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis of COLO 320 using 66338-1-Ig (same clone as 66338-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    WB analysis of fetal human brain using 66338-1-Ig (same clone as 66338-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of mouse colon using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of mouse colon using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of human colon cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of human colon cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP3 Monoclonal antibody (66338-1-Ig)

    IHC staining of human colon cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MMP3 Monoclonal antibody (66338-1-Ig)

    IF Staining of A549 using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (66338-1-Ig, Clone: 1F5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MMP3 Monoclonal antibody, PBS Only

MMP3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66338-1-PBS
Clone No.1F5A9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SL-1, MMP-3, MMP 3, Matrix metalloproteinase-3, EC:3.4.24.17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66338-1-PBS targets MMP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMP3 fusion protein Ag12359 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074869
Gene Symbol MMP3
Gene ID (NCBI) 4314
RRIDAB_2881718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08254
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.