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  • Featured Product
  • KD/KO Validated

MMP7 Polyclonal antibody

MMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 10374-2-AP

Synonyms

MMP 7, Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MMP7 Polyclonal antibody (10374-2-AP)

    WB analysis using 10374-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MMP7 Polyclonal antibody (10374-2-AP)

    WB analysis of human placenta using 10374-2-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MMP7 Polyclonal antibody (10374-2-AP)

    WB analysis using 10374-2-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using MMP7 Polyclonal antibody (10374-2-AP)

    WB analysis of NIH/3T3 using 10374-2-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human pancreas cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human pancreas cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human stomach cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human stomach cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human prostate cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human prostate cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human colon cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human colon cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human colon cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human colon cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human prostate cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IHC staining of human prostate cancer using 10374-2-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IF Staining of human pancreas cancer using 10374-2-AP

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using MMP7 Polyclonal antibody (10374-2-AP)

    IF Staining of human pancreas cancer using 10374-2-AP

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MMP7 Polyclonal antibody (10374-2-AP)

    IF Staining of PC-3 using 10374-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MMP7 antibody (10374-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, human placenta tissue, SKOV-3 cells, NIH/3T3 cells, COLO 320 cells, PC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, human prostate cancer tissue, human colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10374-2-AP targets MMP7 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP7 fusion protein Ag0550 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 7 (matrilysin, uterine)
Calculated molecular weight 29 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC003635
Gene symbol MMP7
Gene ID (NCBI) 4316
RRIDAB_2144452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. This antibody can only recognize the full-length of MMP7.

Protocols

Product Specific Protocols
WB protocol for MMP7 antibody 10374-2-APDownload protocol
IHC protocol for MMP7 antibody 10374-2-APDownload protocol
IF protocol for MMP7 antibody 10374-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

Hypoxic tumor-derived exosomal miR-301a mediates M2 macrophage polarization via PTEN/PI3Kγ to promote pancreatic cancer metastasis.

Authors - Xiaofeng Wang
View Article
humanWB

Cancer Res

Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying β-catenin degradation.

Authors - Rongfa Yuan
View Article
humanIHC

Cancer Res

KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.

Authors - Peng Yuan
View Article
humanWB

Oncogene

Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.

Authors - Q Zhou
View Article
humanWB

Clin Cancer Res

BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.

Authors - Zebing Liu
View Article
human,mouseWB,IHC

Int J Cancer

Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.

Authors - Qingmin Yuan
View Article