Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis using 10374-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10374-2-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10374-2-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 10374-2-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 10374-2-AP (MMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10374-2-AP (MMP7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 10374-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10374-2-AP (MMP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human pancreas cancer using 10374-2-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of human pancreas cancer using 10374-2-AP
Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using 10374-2-AP (MMP7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of PC-3 using 10374-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MMP7 antibody (10374-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

MMP7 Polyclonal antibody

MMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 10374-2-AP

Publications(89)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

MMP 7, Matrix metalloproteinase-7, Matrix metalloproteinase 7, Matrin, Matrilysin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (89)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, human placenta tissue, SKOV-3 cells, NIH/3T3 cells, COLO 320 cells, PC-3 cells
Positive IHC detected in	human pancreas cancer tissue, human prostate cancer tissue, human colon cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human pancreas cancer tissue
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 74 publications below
IHC	See 18 publications below
IF	See 1 publications below

Product Information

10374-2-AP targets MMP7 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MMP7 fusion protein Ag0550 相同性解析による交差性が予測される生物種
Full Name	matrix metallopeptidase 7 (matrilysin, uterine)
Calculated molecular weight	29 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC003635
Gene Symbol	MMP7
Gene ID (NCBI)	4316
RRID	AB_2144452
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P09237
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors. This antibody can only recognize the full-length of MMP7.

Protocols

Product Specific Protocols
WB protocol for MMP7 antibody 10374-2-AP	Download protocol
IHC protocol for MMP7 antibody 10374-2-AP	Download protocol
IF protocol for MMP7 antibody 10374-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Res Hypoxic tumor-derived exosomal miR-301a mediates M2 macrophage polarization via PTEN/PI3Kγ to promote pancreatic cancer metastasis. Authors - Xiaofeng Wang View Article
human	WB	Cancer Res Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying β-catenin degradation. Authors - Rongfa Yuan View Article
human	IHC	Cancer Res KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis. Authors - Peng Yuan View Article
human	WB	Oncogene Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway. Authors - Q Zhou View Article
human	WB	Clin Cancer Res BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs. Authors - Zebing Liu View Article
human,mouse	WB,IHC	Int J Cancer Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway. Authors - Qingmin Yuan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10374-2-AP

WB analysis using 10374-2-AP

WB analysis of NIH/3T3 using 10374-2-AP

WB analysis of human placenta using 10374-2-AP

IHC staining of human pancreas cancer using 10374-2-AP

IHC staining of human pancreas cancer using 10374-2-AP

IHC staining of human prostate cancer using 10374-2-AP

IHC staining of human prostate cancer using 10374-2-AP

IHC staining of human prostate cancer using 10374-2-AP

IHC staining of human colon cancer using 10374-2-AP

IHC staining of human colon cancer using 10374-2-AP

IHC staining of human colon cancer using 10374-2-AP

IHC staining of human colon cancer using 10374-2-AP

IHC staining of human prostate cancer using 10374-2-AP

IHC staining of human stomach cancer using 10374-2-AP

IHC staining of human stomach cancer using 10374-2-AP

IF Staining of human pancreas cancer using 10374-2-AP

IF Staining of human pancreas cancer using 10374-2-AP

IF Staining of PC-3 using 10374-2-AP

MMP7 Polyclonal antibody

MMP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hypoxic tumor-derived exosomal miR-301a mediates M2 macrophage polarization via PTEN/PI3Kγ to promote pancreatic cancer metastasis.

Ubiquitin-like protein FAT10 promotes the invasion and metastasis of hepatocellular carcinoma by modifying β-catenin degradation.

KRAS-NFκB-YY1-miR-489 signaling axis controls pancreatic cancer metastasis.

Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.

BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.

Modification of α2,6-sialylation mediates the invasiveness and tumorigenicity of non-small cell lung cancer cells in vitro and in vivo via Notch1/Hes1/MMPs pathway.