- Featured Product
- KD/KO Validated
MMP8 Polyclonal antibody
MMP8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17874-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, mouse liver tissue |
| Positive IHC detected in | human placenta tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 4 publications below |
Product Information
17874-1-AP targets MMP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MMP8 fusion protein Ag12367 相同性解析による交差性が予測される生物種 |
| Full Name | matrix metallopeptidase 8 (neutrophil collagenase) |
| Calculated molecular weight | 467 aa, 53 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC074988 |
| Gene symbol | MMP8 |
| Gene ID (NCBI) | 4317 |
| RRID | AB_2144582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa, respectively(PMID: 28772283, PMID:15042023).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP8 antibody 17874-1-AP | Download protocol |
| IHC protocol for MMP8 antibody 17874-1-AP | Download protocol |
| IF protocol for MMP8 antibody 17874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Pathog Circumventing Y. pestis Virulence by Early Recruitment of Neutrophils to the Lungs during Pneumonic Plague. | ||
Stem Cell Res Ther Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model. | ||
Am J Pathol Preeclampsia is associated with alterations in DNA methylation of genes involved in collagen metabolism. | ||
Viruses Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice. | ||
Mediators Inflamm Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes. | ||
Exp Eye Res Luteolin ameliorates cornea stromal collagen degradation and inflammatory damage in rats with corneal alkali burn |
