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MMP9 (N-terminal) Polyclonal antibody
MMP9 (N-terminal) Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
718
Cat no : 10375-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 639 publications below |
| IHC | See 85 publications below |
| IF | See 34 publications below |
| CoIP | See 2 publications below |
Product Information
10375-2-AP targets MMP9 (N-terminal) in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | fish, hamster, human, mouse, pig, rabbit, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MMP9 (N-terminal) fusion protein Ag0552 |
| Full Name | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
| Calculated molecular weight | 707 aa, 78 kDa |
| Observed molecular weight | 67 kDa, 92 kDa |
| GenBank accession number | BC006093 |
| Gene symbol | MMP9 |
| Gene ID (NCBI) | 4318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
| IHC protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
| IF protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
| FC protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JCI Insight Matrix metalloproteinase-9 deficiency protects mice from severe influenza A viral infection. | ||
Mol Cancer Alteration of tumor suppressor BMP5 in sporadic colorectal cancer: a genomic and transcriptomic profiling based study. | ||
Cytotechnology Angiopoietin-like protein 1 inhibits epithelial to mesenchymal transition in colorectal cancer cells via suppress Slug expression. | ||
Dose Response Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin. | ||
J Cell Physiol Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3. | ||
Int J Mol Med miR‑16‑2‑3p inhibits cell proliferation and migration and induces apoptosis by targeting PDPK1 in maxillary primordium mesenchymal cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hongxue (Verified Customer) (01-12-2021) | Clear band for WB
 |
FH Kyosuke (Verified Customer) (06-12-2019) | I am working on aneurysm study and use this for IHC staining on mouse aneurysm section. It works very well.
|
FH JIMMY (Verified Customer) (04-15-2019) | This antibody detects MMP9 expressing cells in the spinal cord of C57BL/6 EAE mice. The imaging is quiet clear with this antibody.
|
FH Lucy (Verified Customer) (03-08-2019) | Reacts mainly to human MMP9 but slightly cross-reactive to human MMP2
 |
