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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of fetal human brain using 66049-1-Ig (same clone as 66049-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of rat brain using 66049-1-Ig (same clone as 66049-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of NIH/3T3 using 66049-1-Ig (same clone as 66049-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of RAW 264.7 using 66049-1-Ig (same clone as 66049-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of human kidney using 66049-1-Ig (same clone as 66049-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of HepG2 using 66049-1-Ig (same clone as 66049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of human brain using 66049-1-Ig (same clone as 66049-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of HepG2 using 66049-1-Ig (same clone as 66049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of HEK-293 using 66049-1-Ig (same clone as 66049-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using MMS19 Monoclonal antibody (66049-1-Ig)

    WB analysis of RAW 264.7 using 66049-1-Ig (same clone as 66049-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    IP experiment of mouse brain using 66049-1-Ig (same clone as 66049-1-PBS)

    IP result of anti-MMS19 (IP:66049-1-Ig, 4ug; Detection:66049-1-Ig 1:2000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MMS19 Monoclonal antibody (66049-1-Ig)

    IHC staining of human cervical cancer using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66049-1-Ig(MMS19 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MMS19 Monoclonal antibody (66049-1-Ig)

    IF Staining of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

    Immunofluorescent analysis of fixed Hela cells using 66049-1-Ig (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MMS19 Monoclonal antibody, PBS Only

MMS19 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66049-1-PBS
Clone No.2H6D8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66049-1-PBS targets MMS19 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MMS19 fusion protein Ag8960 相同性解析による交差性が予測される生物種
Full Name MMS19 nucleotide excision repair homolog (S. cerevisiae)
Calculated molecular weight 1030 aa, 113 kDa
Observed molecular weight 103 kDa, 38 kDa
GenBank accession numberBC006575
Gene Symbol MMS19
Gene ID (NCBI) 64210
RRIDAB_11041708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96T76
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.