Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, U2OS cells |
| Positive IHC detected in | human breast cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
24697-1-AP targets MN1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MN1 fusion protein Ag20344 相同性解析による交差性が予測される生物種 |
| Full Name | meningioma (disrupted in balanced translocation) 1 |
| Calculated molecular weight | 1320 aa, 136 kDa |
| Observed molecular weight | 136 kDa |
| GenBank accession number | BC156879 |
| Gene Symbol | MN1 |
| Gene ID (NCBI) | 4330 |
| RRID | AB_2879678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q10571 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MN1 antibody 24697-1-AP | Download protocol |
| IHC protocol for MN1 antibody 24697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Astroblastomas exhibit radial glia stem cell lineages and differential expression of imprinted and X-inactivation escape genes. | ||
EBioMedicine METTL14-mediated epitranscriptome modification of MN1 mRNA promote tumorigenicity and all-trans-retinoic acid resistance in osteosarcoma.
| ||
Acta Neuropathol MN1 immunohistochemistry is a sensitive diagnostic biomarker for primitive CNS tumors with MN1 fusion |