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KD/KO Validated

MNAT1 Polyclonal antibody

Name: MNAT1 Polyclonal antibody
Brand: Proteintech
SKU: 11719-1-AP

MNAT1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11719-1-AP

Synonyms

Cyclin-G1-interacting protein, Cyclin G1 interacting protein, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, CDK7/cyclin H assembly factor

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis using 11719-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11719-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11719-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11719-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11719-1-AP
IP result of anti-MNAT1 (IP:11719-1-AP, 4ug; Detection:11719-1-AP 1:500) with HeLa cells lysate 1200ug.
IF Staining of A375 using 11719-1-AP
Immunofluorescent analysis of (4% PFA) fixed A375 cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 11719-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 11719-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A375 cells, MCF-7 cells, NIH/3T3 cells, A431 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	A375 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 2 publications below

Product Information

11719-1-AP targets MNAT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MNAT1 fusion protein Ag2293 相同性解析による交差性が予測される生物種
Full Name	menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)
Calculated molecular weight	309 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC000820
Gene symbol	MNAT1
Gene ID (NCBI)	4331
RRID	AB_2145054
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MNAT1 antibody 11719-1-AP	Download protocol
IF protocol for MNAT1 antibody 11719-1-AP	Download protocol
IP protocol for MNAT1 antibody 11719-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Int J Biol Sci Therapeutic Targeting of CDK7 Suppresses Tumor Progression in Intrahepatic Cholangiocarcinoma. Authors - Hua-Dong Chen View Article
human	WB,IHC	BMC Cancer MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway. Authors - Chensheng Qiu KD Validated View Article
mouse	WB	Acta Histochem Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice. Authors - Xuexian Liu View Article
mouse	WB	Genes Dev TRF1 uses a noncanonical function of TFIIH to promote telomere replication Authors - Zhe Yang View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MNAT1 Polyclonal antibody

MNAT1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11719-1-AP

WB analysis of NIH/3T3 using 11719-1-AP

WB analysis of A375 using 11719-1-AP

WB analysis of MCF-7 using 11719-1-AP

IP experiment of HeLa using 11719-1-AP

IF Staining of A375 using 11719-1-AP

IF Staining of HeLa using 11719-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Therapeutic Targeting of CDK7 Suppresses Tumor Progression in Intrahepatic Cholangiocarcinoma.

MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway.

Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice.

TRF1 uses a noncanonical function of TFIIH to promote telomere replication