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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MNDA Polyclonal antibody

MNDA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13576-1-AP

Synonyms

PYHIN3

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using MNDA Polyclonal antibody (13576-1-AP)

    WB analysis of K-562 using 13576-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using MNDA Polyclonal antibody (13576-1-AP)

    WB analysis of HL-60 using 13576-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human bladder tissue using MNDA Polyclonal antibody (13576-1-AP)

    WB analysis of human bladder using 13576-1-AP

    human bladder tissue were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MNDA Polyclonal antibody (13576-1-AP)

    IHC staining of human stomach using 13576-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13576-1-AP (MNDA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inK-562 cells, HL-60 cells, human bladder tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13576-1-AP targets MNDA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MNDA fusion protein Ag4476 相同性解析による交差性が予測される生物種
Full Name myeloid cell nuclear differentiation antigen
Calculated molecular weight 407 aa, 46 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC032319
Gene symbol MNDA
Gene ID (NCBI) 4332
RRIDAB_2145071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MNDA antibody 13576-1-APDownload protocol
IHC protocol for MNDA antibody 13576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Mol Med

WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling.

Authors - Chao Sun
View Article