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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MNS1 Polyclonal antibody

MNS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12693-1-AP

Synonyms

meiosis nuclear structural 1, MNS1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using MNS1 Polyclonal antibody (12693-1-AP)

    WB analysis of rat testis using 12693-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12693-1-AP (MNS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using MNS1 Polyclonal antibody (12693-1-AP)

    WB analysis of rat testis using 12693-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12693-1-AP (MNS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MNS1 Polyclonal antibody (12693-1-AP)

    IHC staining of human testis using 12693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12693-1-AP (MNS1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MNS1 Polyclonal antibody (12693-1-AP)

    IHC staining of human testis using 12693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12693-1-AP (MNS1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inrat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12693-1-AP targets MNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MNS1 fusion protein Ag3423 相同性解析による交差性が予測される生物種
Full Name meiosis-specific nuclear structural 1
Calculated molecular weight 495 aa, 61 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC034991
Gene symbol MNS1
Gene ID (NCBI) 55329
RRIDAB_2877875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Meiosis-specific nuclear structural 1 (MNS1), is abundantly expressed in post-meiotic spermatids and is required for proper flagellar formation and function. Male mice deficient in MNS1 display decreased sperm production and are sterile because their sperm are immotile. The microtubules and ODFs of the flagella are disrupted, resulting in abnormal, short sperm tails. In addition, MNS1 is also required for motile ciliary functions.

Protocols

Product Specific Protocols
WB protocol for MNS1 antibody 12693-1-APDownload protocol
IHC protocol for MNS1 antibody 12693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols