MNT Recombinant antibody
MNT Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240550A10
Cat no : 83648-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293T cells, Jurkat cells, A431 cells, HT-29 cells, C6 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 83648-1-RR is MNT Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | MNT fusion protein Ag34529 相同性解析による交差性が予測される生物種 |
| Full Name | MAX binding protein |
| Calculated molecular weight | 582 aa, 62 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC117563 |
| Gene symbol | MNT |
| Gene ID (NCBI) | 4335 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MNT antibody 83648-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
