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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MOCS2B Polyclonal antibody (14416-1-AP)

    WB analysis using 14416-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MOCS2B Polyclonal antibody (14416-1-AP)

    WB analysis of mouse heart using 14416-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MOCS2B Polyclonal antibody (14416-1-AP)

    WB analysis of HeLa using 14416-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MOCS2B Polyclonal antibody (14416-1-AP)

    WB analysis of HepG2 using 14416-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat heart tissue using MOCS2B Polyclonal antibody (14416-1-AP)

    IHC staining of rat heart using 14416-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14416-1-AP (MOCS2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using MOCS2B Polyclonal antibody (14416-1-AP)

    IHC staining of rat heart using 14416-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14416-1-AP (MOCS2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MOCS2B Polyclonal antibody (14416-1-AP)

    IF Staining of HeLa using 14416-1-AP

    Immunofluorescent analysis of HeLa cells using 14416-1-AP (MOCS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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MOCS2B Polyclonal antibody

MOCS2B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14416-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MOCS2, MOCO1-B, MOCO1-A, MOCO1 A, MOCO1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14416-1-AP targets MOCS2B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOCS2B fusion protein Ag5761 相同性解析による交差性が予測される生物種
Full Name molybdenum cofactor synthesis 2
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC046097
Gene Symbol MOCS2
Gene ID (NCBI) 4338
RRIDAB_2250779
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO96033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MOCS2B antibody 14416-1-APDownload protocol
IF protocol for MOCS2B antibody 14416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols