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- KD/KO Validated
MOF Polyclonal antibody
MOF Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 13842-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, rat liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HCT 116 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
13842-1-AP targets MOF in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MOF fusion protein Ag4802 相同性解析による交差性が予測される生物種 |
| Full Name | MYST histone acetyltransferase 1 |
| Calculated molecular weight | 467 aa, 53 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC037773 |
| Gene symbol | KAT8/MOF |
| Gene ID (NCBI) | 84148 |
| RRID | AB_2146894 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MOF, also known as MYST1, KAT8, is a member of the MYST acetyltransferase family, which is homologous to Drosophila males absent on the first (MOF) protein. Deletion of MYST1 in cells leads to genomic instability, spontaneous chromosomal aberration, cell cycle defects, reduced transcription of certain genes and defective DNA damage repair (PMID: 32711345). MYST1 dually localizes to the nucleus and mitochondria of human cancer cells (PMID: 37813994). Western blot analysis detected MOF at an apparent molecular mass of 52 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MOF antibody 13842-1-AP | Download protocol |
| IF protocol for MOF antibody 13842-1-AP | Download protocol |
| IP protocol for MOF antibody 13842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity. | ||
Redox Biol Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.
| ||
Mol Cell Proteomics Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm). | ||
Mol Cell Proteomics Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human. | ||
Cell Physiol Biochem Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis.
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