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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MOG Polyclonal antibody

MOG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28752-1-AP

Synonyms

MOG, MOGIG 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MOG Polyclonal antibody (28752-1-AP)

    WB analysis using 28752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28752-1-AP (MOG antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MOG Polyclonal antibody (28752-1-AP)

    IHC staining of mouse brain using 28752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28752-1-AP (MOG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using MOG Polyclonal antibody (28752-1-AP)

    IHC staining of rat brain using 28752-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 28752-1-AP (MOG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28752-1-AP targets MOG in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOG fusion protein Ag30377 相同性解析による交差性が予測される生物種
Full Name myelin oligodendrocyte glycoprotein
Calculated molecular weight 295 aa, 34 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC035938
Gene symbol MOG
Gene ID (NCBI) 4340
RRIDAB_2881208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MOG antibody 28752-1-APDownload protocol
IHC protocol for MOG antibody 28752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols