Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human small intestine tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
17859-1-AP targets GCS1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GCS1 fusion protein Ag12321 相同性解析による交差性が予測される生物種 |
| Full Name | mannosyl-oligosaccharide glucosidase |
| Calculated molecular weight | 837 aa, 92 kDa |
| Observed molecular weight | 80-92 kDa |
| GenBank accession number | BC028337 |
| Gene Symbol | GCS1 |
| Gene ID (NCBI) | 7841 |
| RRID | AB_10694270 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13724 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MOGS (Mannosyl-oligosaccharide glucosidase), also known as GCS1, is the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor (PMID: 38187061). The resulting Glc(2)-Man(9)-GlcNAc(2) intermediate is then further modified by glucosidase II and several ER- and Golgi-resident mannosidases and glycosyltransferases, finally yielding a complex array of glycan structures (PMID: 3896128). MOGS has two isoforms, and it is located in the lumen of the endoplasmic reticulum.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GCS1 antibody 17859-1-AP | Download protocol |
| IHC protocol for GCS1 antibody 17859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins | ||
Int J Mol Sci The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts. | ||
Front Genet Subtypes analysis and prognostic model construction based on lysosome-related genes in colon adenocarcinoma | ||
Am J Physiol Cell Physiol Hydrogen Sulfide Accumulates LDL Receptor Precursor via Downregulating PCSK9 in HepG2 Cells. | ||
Neurogenetics Identification and characterization of novel mutations in MOGS in a Chinese patient with infantile spams. |