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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MON1B Polyclonal antibody (17638-1-AP)

    WB analysis using 17638-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17638-1-AP (MON1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MON1B Polyclonal antibody (17638-1-AP)

    WB analysis of HeLa using 17638-1-AP

    WB result of MON1B antibody (17638-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected HeLa cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MON1B Polyclonal antibody (17638-1-AP)

    IF Staining of HeLa using 17638-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (17638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

MON1B Polyclonal antibody

MON1B Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17638-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SAND2, KIAA0872, HSV-I stimulating-related protein, HSV-1 stimulation-related gene 1 protein, HSRG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17638-1-AP targets MON1B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MON1B fusion protein Ag9920 相同性解析による交差性が予測される生物種
Full Name MON1 homolog B (yeast)
Calculated molecular weight 547 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC024277
Gene Symbol MON1B
Gene ID (NCBI) 22879
RRIDAB_2918054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L1V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).

Protocols

Product Specific Protocols
WB protocol for MON1B antibody 17638-1-APDownload protocol
IF protocol for MON1B antibody 17638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Res

Numb regulates vesicular docking for homotypic fusion of early endosomes via membrane recruitment of Mon1b.

Authors - Ximing Shao
  • KD Validated
View Article