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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MORC3 Monoclonal antibody

MORC3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E1E1

Cat no : 68370-1-Ig

Synonyms

KIAA0136, MORC3, NXP2, ZCW5, ZCWCC3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MORC3 Monoclonal antibody (68370-1-Ig)

    WB analysis using 68370-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68370-1-Ig (MORC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using MORC3 Monoclonal antibody (68370-1-Ig)

    WB analysis using 68370-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68370-1-Ig (MORC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68370-1-Ig targets MORC3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MORC3 fusion protein Ag21867 相同性解析による交差性が予測される生物種
Full Name MORC family CW-type zinc finger 3
Calculated molecular weight 939 aa, 107 kDa
Observed molecular weight130 kDa
GenBank accession numberBC132731
Gene symbol MORC3
Gene ID (NCBI) 23515
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.

Protocols

Product Specific Protocols
WB protocol for MORC3 antibody 68370-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols