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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MORF4L1 Polyclonal antibody

MORF4L1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55257-1-AP

Synonyms

Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MORF4L1 Polyclonal antibody (55257-1-AP)

    WB analysis of HEK-293 using 55257-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    WB analysis of mouse brain using 55257-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    WB analysis of mouse brain using 55257-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MORF4L1 Polyclonal antibody (55257-1-AP)

    WB analysis of HEK-293 using 55257-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MORF4L1 Polyclonal antibody (55257-1-AP)

    WB analysis of A549 using 55257-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using MORF4L1 Polyclonal antibody (55257-1-AP)

    WB analysis of A549 using 55257-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MORF4L1 Polyclonal antibody (55257-1-AP)

    IP experiment of HEK-293 using 55257-1-AP

    IP result of anti-MORF4L1 (IP:55257-1-AP, 4ug; Detection:55257-1-AP 1:800) with HEK-293 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human testis using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human testis using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human testis using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human testis using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human testis using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human testis using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MORF4L1 Polyclonal antibody (55257-1-AP)

    IHC staining of human skin using 55257-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55257-1-AP targets MORF4L1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name mortality factor 4 like 1
Calculated molecular weight 41 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberNM_006791
Gene symbol MORF4L1
Gene ID (NCBI) 10933
RRIDAB_10836938
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MORF4L1, also named as MRG15, belongs to the MRG family. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. (PMID:14966270) This antibody has no cross-reaction to MORF4L2.

Protocols

Product Specific Protocols
WB protocol for MORF4L1 antibody 55257-1-APDownload protocol
IHC protocol for MORF4L1 antibody 55257-1-APDownload protocol
IP protocol for MORF4L1 antibody 55257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols