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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MOSC2 Recombinant antibody (83705-2-RR)

    WB analysis using 83705-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MOSC2 Recombinant antibody (83705-2-RR)

    WB analysis using 83705-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83705-2-RR (MOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MOSC2 Recombinant antibody (83705-2-RR)

    IHC staining of human stomach cancer using 83705-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MOSC2 Recombinant antibody (83705-2-RR)

    IHC staining of human stomach cancer using 83705-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83705-2-RR (MOSC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HeLa cells using MOSC2 Recombinant antibody (83705-2-RR)

    FC experiment of HeLa using 83705-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MOSC2 Recombinant antibody (83705-2-RR, Clone:240747G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83705-2-RR

    Affinity and Kinetic Characterization of 83705-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83705-2-RR against Human MOSC2 were performed. The affinity constant is 0.473 nM.

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MOSC2 Recombinant antibody

MOSC2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83705-2-RR
Clone No.240747G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Molybdenum cofactor sulfurase C-terminal domain-containing protein 2, Moco sulfurase C-terminal domain-containing protein 2, Mitochondrial amidoxime reducing component 2, mARC2, EC:1.7.-.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HUVEC cells, HEK-293 cells, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83705-2-RR targets MOSC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MOSC2 fusion protein Ag20694 相同性解析による交差性が予測される生物種
Full Name MOCO sulphurase C-terminal domain containing 2
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC011973
Gene Symbol MOSC2
Gene ID (NCBI) 54996
RRIDAB_3671307
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969Z3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MOSC domain-containing protein 2 (also known as MOSC2), also known as MARC2, is a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. Also, MOSC2 may be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. The reductase activity is regulated under adipogenic conditions, and down-regulation of the terminal component MOSC2 resulted in decreased lipid synthesis, suggesting a possible physiological role of this enzyme system and its component MOSC2 in lipogenesis(PMID: 22203676).

Protocols

Product Specific Protocols
WB protocol for MOSC2 antibody 83705-2-RRDownload protocol
IHC protocol for MOSC2 antibody 83705-2-RRDownload protocol
FC protocol for MOSC2 antibody 83705-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols