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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MPG Polyclonal antibody, PBS Only

MPG Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11481-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MPG Polyclonal antibody (11481-2-AP)

    WB analysis of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MPG Polyclonal antibody (11481-2-AP)

    WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using MPG Polyclonal antibody (11481-2-AP)

    WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using MPG Polyclonal antibody (11481-2-AP)

    WB analysis of Jurkat using 11481-2-AP (same clone as 11481-2-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MPG Polyclonal antibody (11481-2-AP)

    IP experiment of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

    IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MPG Polyclonal antibody (11481-2-AP)

    IHC staining of human prostate cancer using 11481-2-AP (same clone as 11481-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11481-2-PBS targets MPG in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPG fusion protein Ag2034 相同性解析による交差性が予測される生物種
Full Name N-methylpurine-DNA glycosylase
Calculated molecular weight 293 aa, 32 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC014991
Gene symbol MPG
Gene ID (NCBI) 4350
RRIDAB_2282144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].