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  • Featured Product
  • KD/KO Validated

MPO Monoclonal antibody, PBS Only

MPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C11F6

Cat no : 66177-1-PBS

Synonyms

myeloperoxidase, EC:1.11.2.2, 4C11F6

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using MPO Monoclonal antibody (66177-1-Ig)

    WB analysis of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66177-1-Ig (MPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human liver using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IHC staining of human tonsillitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66177-1-Ig (MPO antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using MPO Monoclonal antibody (66177-1-Ig)

    IF Staining of human appendicitis using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HL-60 cells using MPO Monoclonal antibody (66177-1-Ig)

    IF Staining of HL-60 using 66177-1-Ig (same clone as 66177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (66177-1-Ig, Clone: 4C11F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66177-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66177-1-PBS targets MPO in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen MPO fusion protein Ag17564 相同性解析による交差性が予測される生物種
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
RRIDAB_2881572
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.