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  • Featured Product
  • KD/KO Validated

MPO Recombinant antibody

MPO Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3J17

Cat no : 81610-1-RR

Synonyms

myeloperoxidase, EC:1.11.2.2, 3J17

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using MPO Recombinant antibody (81610-1-RR)

    WB analysis of HL-60 using 81610-1-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human saliva using MPO Recombinant antibody (81610-1-RR)

    WB analysis of human saliva using 81610-1-RR

    human saliva were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using MPO Recombinant antibody (81610-1-RR)

    WB analysis of HL-60 using 81610-1-RR

    WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells.

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Recombinant antibody (81610-1-RR)

    IHC staining of human liver using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MPO Recombinant antibody (81610-1-RR)

    IHC staining of human liver using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MPO Recombinant antibody (81610-1-RR)

    IHC staining of human colon cancer using 81610-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81610-1-RR (MPO antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HL-60 cells using MPO Recombinant antibody (81610-1-RR)

    IF Staining of HL-60 using 81610-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81610-1-RR

    Affinity and Kinetic Characterization of 81610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

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Tested Applications

Positive WB detected inHL-60 cells, human saliva
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81610-1-RR targets MPO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MPO fusion protein Ag17564 相同性解析による交差性が予測される生物種
Full Name myeloperoxidase
Calculated molecular weight 745 aa, 84 kDa
Observed molecular weight 59 kDa, 90 kDa
GenBank accession numberBC130476
Gene symbol MPO
Gene ID (NCBI) 4353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MPO antibody 81610-1-RRDownload protocol
IHC protocol for MPO antibody 81610-1-RRDownload protocol
IF protocol for MPO antibody 81610-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols