MPO Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP00321-2, Human MPO Recombinant Matched Antibody Pair - PBS only. 83282-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0511. 83282-5-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

The mean MPO concentration was determined to be 73,583.3 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

Serum of sixteen individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 169.9 ng/mL with a range of 54.5 - 419.2 ng/mL.

Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean MPO concentration was determined to be 6.6 ng/mL in unstimulated PBMC supernatant, 20.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean MPO concentration was determined to be 7.2 ng/mL in unstimulated PBMC supernatant, 28.2 ng/mL in PHA stimulated PBMC supernatant.

Urine of six individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 2,249.9 pg/mL with a range of 294.1 - 6,991.1 pg/mL.

Saliva of eight individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 314.7 ng/mL with a range of 6.3 - 1,446.1 ng/mL.