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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPP2 Polyclonal antibody (13589-1-AP)

    WB analysis using 13589-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using MPP2 Polyclonal antibody (13589-1-AP)

    WB analysis of mouse eye using 13589-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 13589-1-AP (MPP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse eye tissue using MPP2 Polyclonal antibody (13589-1-AP)

    IP experiment of mouse eye using 13589-1-AP

    IP result of anti-MPP2 (IP:13589-1-AP, 4ug; Detection:13589-1-AP 1:2000) with mouse eye tissue lysate 2260 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPP2 Polyclonal antibody (13589-1-AP)

    IHC staining of mouse brain using 13589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MPP2 Polyclonal antibody (13589-1-AP)

    IHC staining of mouse brain using 13589-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13589-1-AP (MPP2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MPP2 Polyclonal antibody

MPP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13589-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

Discs large homolog 2, DLG2, MAGUK p55 subfamily member 2, MPP2, Protein MPP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue, mouse retina tissue
Positive IP detected inmouse eye tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13589-1-AP targets MPP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPP2 fusion protein Ag4223 相同性解析による交差性が予測される生物種
Full Name membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Calculated molecular weight 576 aa, 65 kDa
Observed molecular weight 65 kDa, 60 kDa
GenBank accession numberBC030287
Gene Symbol MPP2
Gene ID (NCBI) 4355
RRIDAB_2935434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14168
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Membrane protein palmitoylated 2 (MPP2) is a member of the membrane-associated guanylate kinase (MAGUK) family. MPPs can form diverse protein complexes at the cell membranes and play an important role in cell structure, polarity and cell adhesion. MPP2 is a novel postsynaptic scaffold protein that binds to the synaptic cell adhesion molecule SynCAM1. MPP2 also is a component of AMPA receptor-associated protein complexes.

Protocols

Product Specific Protocols
WB protocol for MPP2 antibody 13589-1-APDownload protocol
IHC protocol for MPP2 antibody 13589-1-APDownload protocol
IP protocol for MPP2 antibody 13589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols