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- KD/KO Validated
MPP5 Polyclonal antibody
MPP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17710-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, human brain tissue, Y79 cells, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 7 publications below |
| IF | See 16 publications below |
| IP | See 1 publications below |
Product Information
17710-1-AP targets MPP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MPP5 fusion protein Ag12010 相同性解析による交差性が予測される生物種 |
| Full Name | membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) |
| Calculated molecular weight | 675 aa, 77 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC095485 |
| Gene symbol | MPP5 |
| Gene ID (NCBI) | 64398 |
| RRID | AB_2282012 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPP5 antibody 17710-1-AP | Download protocol |
| IHC protocol for MPP5 antibody 17710-1-AP | Download protocol |
| IF protocol for MPP5 antibody 17710-1-AP | Download protocol |
| IP protocol for MPP5 antibody 17710-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate. | ||
Neuron Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells. | ||
Dev Cell Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm | ||
Cell Death Differ The evolutionary history of the polyQ tract in huntingtin sheds light on its functional pro-neural activities. | ||
J Am Soc Nephrol Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier. |
