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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MPP6 Polyclonal antibody

MPP6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11575-1-AP

Synonyms

MAGUK p55 subfamily member 6, MPP6, p55T, PALS2, VAM 1, VAM1, Veli associated MAGUK 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPP6 Polyclonal antibody (11575-1-AP)

    WB analysis using 11575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11575-1-AP (MPP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MPP6 Polyclonal antibody (11575-1-AP)

    IHC staining of mouse testis using 11575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11575-1-AP (MPP6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MPP6 Polyclonal antibody (11575-1-AP)

    IHC staining of mouse testis using 11575-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11575-1-AP (MPP6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11575-1-AP targets MPP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPP6 fusion protein Ag2158 相同性解析による交差性が予測される生物種
Full Name membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
Calculated molecular weight 540 aa, 61 kDa
Observed molecular weight 55-61 kDa
GenBank accession numberBC023638
Gene symbol MPP6
Gene ID (NCBI) 51678
RRIDAB_2250848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MPP6 antibody 11575-1-APDownload protocol
IHC protocol for MPP6 antibody 11575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

Elevated MPP6 expression correlates with an unfavorable prognosis, angiogenesis and immune evasion in hepatocellular carcinoma

Authors - Qianqian Cheng
View Article