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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MPP8 Recombinant antibody

MPP8 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242205B5

Cat no : 84719-4-RR

Synonyms

MPHOSPH8, Two hybrid-associated protein 3 with RanBPM, Twa3, M-phase phosphoprotein 8, HSMPP8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MPP8 Recombinant antibody (84719-4-RR)

    WB analysis using 84719-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84719-4-RR (MPP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84719-4-RR targets MPP8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MPP8 fusion protein Ag10268 相同性解析による交差性が予測される生物種
Full Name M-phase phosphoprotein 8
Calculated molecular weight 860 aa, 97 kDa
Observed molecular weight 97-105 kDa
GenBank accession numberBC003542
Gene symbol MPP8
Gene ID (NCBI) 54737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a)

Protocols

Product Specific Protocols
WB protocol for MPP8 antibody 84719-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols