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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MPPED1 Polyclonal antibody

MPPED1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13677-1-AP

Synonyms

Metallophosphoesterase domain-containing protein 1, FAM1A, EC:3.1.-.-, C22orf1, 239AB

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    WB analysis of mouse brain using 13677-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    WB analysis of rat brain using 13677-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    IHC staining of human pancreas using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    IHC staining of human pancreas using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    IHC staining of human brain using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13677-1-AP (MPPED1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    IHC staining of human brain using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13677-1-AP (MPPED1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    IHC staining of human heart using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13677-1-AP (MPPED1 Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MPPED1 Polyclonal antibody (13677-1-AP)

    IHC staining of human heart using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13677-1-AP (MPPED1 Antibody) at dilution of 1:50(under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MPPED1 Polyclonal antibody (13677-1-AP)

    IF Staining of HeLa using 13677-1-AP

    Immunofluorescent analysis of Hela cells, using MPPED1 antibody 13677-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue, human brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13677-1-AP targets MPPED1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPPED1 fusion protein Ag4672 相同性解析による交差性が予測される生物種
Full Name metallophosphoesterase domain containing 1
Calculated molecular weight 326 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC028035
Gene symbol MPPED1
Gene ID (NCBI) 758
RRIDAB_10644336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MPPED1 antibody 13677-1-APDownload protocol
IHC protocol for MPPED1 antibody 13677-1-APDownload protocol
IF protocol for MPPED1 antibody 13677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols