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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MPPED2 Polyclonal antibody

MPPED2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13270-1-AP

Synonyms

Metallophosphoesterase domain-containing protein 2, FAM1B, EC:3.1.-.-, C11orf8, 239FB

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using MPPED2 Polyclonal antibody (13270-1-AP)

    WB analysis of human brain using 13270-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13270-1-AP (MPPED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using MPPED2 Polyclonal antibody (13270-1-AP)

    IHC staining of human brain using 13270-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13270-1-AP (MPPED2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MPPED2 Polyclonal antibody (13270-1-AP)

    IHC staining of human brain using 13270-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13270-1-AP (MPPED2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MPPED2 Polyclonal antibody (13270-1-AP)

    IF Staining of HepG2 using 13270-1-AP

    Immunofluorescent analysis of HepG2 cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MPPED2 Polyclonal antibody (13270-1-AP)

    IF Staining of HeLa using 13270-1-AP

    Immunofluorescent analysis of Hela cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MPPED2 Polyclonal antibody (13270-1-AP)

    IF Staining of HeLa using 13270-1-AP

    Immunofluorescent analysis of Hela cells, using MPPED2 antibody 13270-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13270-1-AP targets MPPED2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPPED2 fusion protein Ag3963 相同性解析による交差性が予測される生物種
Full Name metallophosphoesterase domain containing 2
Calculated molecular weight 294 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC031582
Gene symbol MPPED2
Gene ID (NCBI) 744
RRIDAB_2144517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MPPED2 antibody 13270-1-APDownload protocol
IHC protocol for MPPED2 antibody 13270-1-APDownload protocol
IF protocol for MPPED2 antibody 13270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols