Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
60103-1-Ig targets MPV17 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MPV17 fusion protein Ag0312 相同性解析による交差性が予測される生物種 |
| Full Name | MpV17 mitochondrial inner membrane protein |
| Calculated molecular weight | 20 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC001115 |
| Gene Symbol | MPV17 |
| Gene ID (NCBI) | 4358 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic Acid/Ammonium Sulfate Precipitation |
| UNIPROT ID | P39210 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MPV17, also termed as SYM1, encodes an inner mitochondrial membrane protein. It’s potential candidate that may account for the mitochondrial (mt) DNA depletion syndromes (MDDS) characterized by a severe, tissue-specific decrease of mtDNA copy number and ultimate organ failure. It’s a human ortholog of the mouse kidney disease gene Mpv17. Absence or malfunction of MPV17 causes oxidative phosphorylation (OXPHOS) failure and mtDNA depletion, not only in affected individuals but also in Mpv17-/- mice. MPV17 contribute to ROS homeostasis, but the downstream signaling is unknown yet. Catalog#60103-1-Ig is a mouse monoclonal antibody raised against the full-length of human MPV17.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MPV17 antibody 60103-1-Ig | Download protocol |
| IHC protocol for MPV17 antibody 60103-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Res Notes A monoclonal antibody raised against bacterially expressed MPV17 sequences shows peroxisomal, endosomal and lysosomal localisation in U2OS cells. |