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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MPZL3 Polyclonal antibody

MPZL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25513-1-AP

Synonyms

MPZL3, myelin protein zero like 3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using MPZL3 Polyclonal antibody (25513-1-AP)

    WB analysis of mouse liver using 25513-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25513-1-AP (MPZL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MPZL3 Polyclonal antibody (25513-1-AP)

    WB analysis of mouse brain using 25513-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25513-1-AP (MPZL3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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Tested Applications

Positive WB detected inmouse liver tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25513-1-AP is MPZL3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MPZL3 fusion protein Ag21905 相同性解析による交差性が予測される生物種
Full Name myelin protein zero-like 3
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight 70 kDa, 28 kDa
GenBank accession numberBC113586
Gene symbol MPZL3
Gene ID (NCBI) 196264
RRIDAB_2880111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MPZL3 (Myelin protein zero-like protein 3) is a member of the myelin P0 protein family. The human MPZL3 gene is located at chromosome 11q23.3, and encodes a 235-amino acid polypeptide with an Immunoglobulin V-type (IgV) domain. MPZL3 protein is a membrane adhesion protein. It may be involved in immune function (PMID: 19054061). This polyclonal antibody raised against 32-235aa of human MPZL3 reveals bands of 28 kDa and 70 kDa, which could be endogenous MPZL3 of unmodified form and post-translational modified form, respectively (PMID: 17273165).

Protocols

Product Specific Protocols
WB protocol for MPZL3 antibody 25513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

Met-HER3 crosstalk supports proliferation via MPZL3 in MET-amplified cancer cells.

Authors - Yaakov E Stern
View Article