Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human testis tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
19512-1-AP targets MRAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | melanocortin 2 receptor accessory protein |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | NM_178817 |
| Gene Symbol | MRAP |
| Gene ID (NCBI) | 56246 |
| RRID | AB_10694272 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TCY5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRAP, also named as C21orf61, FALP and B27, is required for MC2R expression in certain cell types. MRAP is involved in the processing, trafficking or function of MC2R. MRAP may be involved in the intracellular trafficking pathways in adipocyte cells. Mutation of MRAP will cause glucocorticoid deficiency type 2 (GCCD2) which known as familial glucocorticoid deficiency type 2 (FGD2). The antibody can recognize the isoform1,isoform2 and isoform4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRAP antibody 19512-1-AP | Download protocol |
| IHC protocol for MRAP antibody 19512-1-AP | Download protocol |
| IF protocol for MRAP antibody 19512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Tissue Res ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling. |